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According to some accounts, such as "The Klepton Parasites" (comic story), John and Gillian were real & were grandchildren of the Doctor. According to other accounts, such as "The Land of Happy Endings" (comic story), they existed only in the Doctor's dreams. According to "Conundrum" (novel), they were imaginary creations of the Master of the Land of Fiction (who is not the same as the Time Lord known as the Master).

Whether they're real or not depends on which (if any) of these accounts are accepted.