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Amy became pregnant by Rory very soon (no more than a few hours) after the end of Series 5. As revealed in "A Good Man Goes to War", Melody Pond (River Song) was conceived in the TARDIS, while travelling through the time vortex on the Ponds' wedding night. The Series 5 finalé, "The Big Bang", closed with them about to depart on that trip in the TARDIS after their wedding reception.